Regulatory

Part:BBa_K606011

Designed by: Cyrille Pauthenier   Group: iGEM11_Paris_Bettencourt   (2011-07-07)

IPTG/Lactose inducible expression casette for B. Subtilis

This part in an IPTG/Lactose inducible expression casette for Bacillus Subtilis. It is build to have a slow and controlable response with the quantity of IPTG introduced in the medium.


Usage and Biology

Control gene expression with IPTG in B. subtilis strains.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
regulator
Parameters
negative_regulators