Composite
Part:BBa_K604000
Designed by: Ruojun Wang Group: iGEM11_Fudan (2011-10-04)
autoinducer synthetase rhlI and GFP under PyeaR
The pathway detects nitrates in the medium; if KNO3 is present, the circuit is activated, RhlI produces the AHL (C4-homoserine lactone) and TetR is synthesized to block another pathway. GFP is emitted to show the start of transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 787
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2115
[edit]
Categories
Parameters
None |