Composite

Part:BBa_K604000

Designed by: Ruojun Wang   Group: iGEM11_Fudan   (2011-10-04)

autoinducer synthetase rhlI and GFP under PyeaR

The pathway detects nitrates in the medium; if KNO3 is present, the circuit is activated, RhlI produces the AHL (C4-homoserine lactone) and TetR is synthesized to block another pathway. GFP is emitted to show the start of transcription.

Barplot.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 787
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2115


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Categories
Parameters
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