Generator

Part:BBa_K602015

Designed by: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh   Group: iGEM11_Osaka   (2011-10-27)

D. radiodurans PprI+PprA expression device

D. radiodurans PprI and PprA, each with own LacI promoter and RBS. Constitutively expresses these two proteins and should increase DNA damage repair capability and radioresistance of host E. coli.

NOTE: There is a double terminator (BBa_B0015) between the PprI CDS (K602000) and the second LacI promoter. The sequence of subparts shown below is NOT accurate until editing of composite parts become available.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None