Measurement

Part:BBa_K602014

Designed by: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh   Group: iGEM11_Osaka   (2011-10-05)


LacI promoter with CrtEBI reporter

Lycopene biosynthesis genes CrtEBI (with RBS) under control of the LacI promoter. The promoter is constitutively on and hence the cells should turn orange-red due to production of lycopene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2182
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1718
    Illegal NgoMIV site found at 1848
    Illegal AgeI site found at 933
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None