Generator

Part:BBa_K602011

Designed by: Toshiyuki Otake, Youfeng Lin, Shuhei Yasumoto, Takahiro Saka, Rie Takino, Shaothing Teoh   Group: iGEM11_Osaka   (2011-10-05)

D. radiodurans radioresistance genes expression device

D. radiodurans PprI, PprA, PprM and RecA attached downstream of LacI constitutive promoter and ribosome binding site. Constitutively expresses these 4 proteins and should increase DNA damage repair capability and radioresistance of host E. coli.

Note that as there is no terminator between each CDS, each transcript will contain all four genes as well as their respective ribosome binding sites. Assuming equal binding to each RBS, genes that come later in the sequence are expected to be translated more frequently (RecA > PprM > PprA > PprI) due to ribosome read-through.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2829
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3503
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None