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Part:BBa_K602004

Designed by: Shao Thing TEOH   Group: iGEM11_Osaka   (2011-10-05)


PprI expression device

D. radiodurans PprI with LacI constitutive promoter and ribosome binding site attached upstream. Constitutively expresses PprI. Should increase DNA damage repair capability and radioresistance of host E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 730
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 363
    Illegal NgoMIV site found at 736
    Illegal NgoMIV site found at 1238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 783
    Illegal SapI.rc site found at 866


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