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Sample In stock
Experience: Works
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Device

Part:BBa_K598025

Designed by: Yan GONG, Xiaowei YAN   Group: iGEM11_pku_riboclamp   (2011-09-28)

Bistable Switch with TPP Hammerhead Ribozyme 2.5 and Theophyline Riboswitch N8-3

The down-regulating TPP ribozyme TPP 2.5 and Theo N8-3 affect the green-red ratio of bistable switch by regulating CI434 and CI expression respectively in this part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1709
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2870


For the full characterisation of the device, please refer to BBa_K598002

[edit]
Categories
Parameters
n/aBistable Switch with TPP Hammerhead Ribozyme 2.5 and Theophyline Riboswitch N8-3