Device

Part:BBa_K598009

Designed by: Siyang HAO, Zhenrun ZHANG   Group: iGEM11_Peking_R   (2011-09-27)

pBAD+Theophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015

This is a measurement with a theophylline responsive riboswitch and reporter gene, GFP. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP measured by GFP fluorescence intensity. In this part, the theophylline responsive riboswitch contains an original ribosome binding site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 869


For the full characterisation of the device, please refer to BBa_K598005

[edit]
Categories
Parameters
chassisE. coli DH5α
ligandstheophylline
n/apBAD+Theophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015
rbsmedium
resistanceChloramphenicol