Reporter

Part:BBa_K598008

Designed by: Yangyang ZHAO, Yan GONG   Group: iGEM11_pku_riboclamp   (2011-09-28)

TPP Up-regulated Hammerhead Ribozyme 1.20 with Native RBS+E0040+B0015

This is a basic part that consists of thiamine pyrophosphate (TPP) ligand responsive hammerhead ribozyme numbered 2.5 with native RBS (AAGGAGAT), BBa_E0040 and BBa_B0015.(fig.1)

Figure 1 Construction of TPP UP-regulated Hammerhead Ribozyme 1.20 with Native RBS+E0040+B0015. This part consists of TPP ribozyme 1.20 with native RBS (AAGGAGAT), BBa_E0040 and BBa_B0015.It is obtained by PCR from TPP-HHAz 1.20 [1] which is kindly provided by Markus Wieland et al., and then inserted into pSB1C3 through standard assembly.

This part is obtained by PCR from TPP-HHAZ 1.20 [1] which is kindly provided by Markus Wieland et. al., and then inserted into pSB1C3 through standard assembly.

Reference

[1] Markus Wieland, Armin Benz, Benedikt Klauser, and Jörg S. Hartig. (2009). Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121, 2753-2756


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 794


[edit]
Categories
Parameters
n/aTPP Up-regulated Hammerhead Ribozyme 1.20 with Native RBS+E0040+B0015