Reporter
Part:BBa_K598008
Designed by: Yangyang ZHAO, Yan GONG Group: iGEM11_Peking_R (2011-09-28)
TPP Up-regulated Hammerhead Ribozyme 1.20 with Native RBS+E0040+B0015
This is a basic part that consists of thiamine pyrophosphate (TPP) ligand responsive hammerhead ribozyme numbered 2.5 with native RBS (AAGGAGAT), BBa_E0040 and BBa_B0015.(fig.1)
This part is obtained by PCR from TPP-HHAZ 1.20 [1] which is kindly provided by Markus Wieland et. al., and then inserted into pSB1C3 through standard assembly.
Reference
[1] Markus Wieland, Armin Benz, Benedikt Klauser, and Jörg S. Hartig. (2009). Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121, 2753-2756
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 794
[edit]
Categories
Parameters
n/a | TPP Up-regulated Hammerhead Ribozyme 1.20 with Native RBS+E0040+B0015 |