Reporter

Part:BBa_K598007

Designed by: Siyang HAO   Group: iGEM11_pku_riboclamp   (2011-09-27)

Theophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015 This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP measured by GFP fluorescence intensity. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to modulate other downstream gene expression. In this part, the theophylline responsive riboswitch contains an original ribosome binding site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 731


For the full characterisation of the device, please refer to BBa_K598005

[edit]
Categories
Parameters
chassisE. coli DH5α
functionused to test the ability of theophylline responsive riboswitch by GFP fluorescence intensity
ligandstheophylline
n/aTheophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015
rbsmedium
resistanceChloramphenicol