RNA

Part:BBa_K598003

Designed by: Yangyang ZHAO, Yan GONG   Group: iGEM11_pku_riboclamp   (2011-09-27)

TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS

It's a basic part including only a TPP ligand responsive ribozyme numbered 2.5, with the native RBS （AAGGAGAT） This part is a thiamine pyrophosphate (TPP) ligand responsive ribozyme numbered 2.5 with native RBS (AAGGAGAT), which can inhibit gene expression responding to TPP. Although there are standard restriction sites in this part, attaching upstream of target gene can only be operated by site-directed mutation. Because the spacing between RBS and start code of target gene after standard assembly is not appropriate.

This part is obtained by PCR from inactive TPP-HHAZ 2.5 [1]which is kindly provided by Markus Wieland et. al., and then inserted into pSB1C3 through standard assembly.


Reference

[1] Markus Wieland, Armin Benz, Benedikt Klauser, and Jörg S. Hartig. (2009). Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121, 2753-2756


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
functionresponse to various concerbtration of TPP and repress gene expression
n/aTPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS
rbsstrong