DNA

Part:BBa_K596002

Designed by: Ebuzer Kalyoncu   Group: iGEM11_Bilkent_UNAM_Turkey   (2011-07-14)


Nitroreductase optimized for B.subtilis

Nitroreductase is a protein and it removes nitro functional group make it less toxic molecules; such as from TNT to monoaminodinitrotoluene(ADNT), 2,4-dinitrotoluene(DNT).Enterobacter cloacae oxygen-insensitive NAD(P)H nitroreductase (nfsI) gene is our basic gene

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 675
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 676
    Illegal PstI site found at 690
    Illegal NotI site found at 7
    Illegal NotI site found at 683
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 676
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 676
    Illegal PstI site found at 690
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None