Coding
raiI

Part:BBa_K594007

Designed by: Yu Xiaoyan   Group: iGEM11_OUC-China   (2011-10-01)


a luxI homologous AHL synthetase from Rhzobium etli CFN 42

RaiI is responsible for the synthesis of 3-OH-C8-HSL, a kind of AHL. The rai system in Rhzobium etli CFN42 can take the example of Rhizobium leguminosarum bv. vicae. RaiI is positively autoregulated by RaiR and 3-OH-C8-HSL but is also induced to a lesser extent by 3-OH-C14:1-HSL(produced by cinI) and 3-oxo-C8-HSL (produced by traI). These observations help explain the effect of cinRI mutations on the production of some of the short-chain AHLs and also suggest that an additional 3-oxo-C8-HSL-producing locus may contribute to regulation of raiRI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 28
    Illegal XhoI site found at 583
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 403
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 31


[edit]
Categories
Parameters
None