Measurement
Part:BBa_K592028
Designed by: Lei Sun Group: iGEM11_Uppsala-Sweden (2011-09-21)
T5-lac promoter-B0032-YFP
The promoter T5-lac (BBa_K592008) tested for promoter strength coupled with YFP (BBa_E0034). This construct should be cloned into pSB3K3 plasmid to measure the YFP output and thereby determine the promoter strength. Theoretically, the promoter should be well-repressed in LacIq strain but inducible by high concentrations of IPTG.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |