Measurement

Part:BBa_K592028

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-21)


T5-lac promoter-B0032-YFP

The promoter T5-lac (BBa_K592008) tested for promoter strength coupled with YFP (BBa_E0034). This construct should be cloned into pSB3K3 plasmid to measure the YFP output and thereby determine the promoter strength. Theoretically, the promoter should be well-repressed in LacIq strain but inducible by high concentrations of IPTG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None