cjBlue Green Coding Device
This part is constitutively expressed BBa_K592011, the cjBlue chromoprotein. The color development is slow, it is readily visible to naked eye after 24-48 hours of incubation.
Escherichia coli constitutively expressing cjBlue (BBa_K592011).
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
2017 Team NYU Abu Dhabi attempted to express cjBlue in DH5-α using composite part BBa_K592022. Our assembly and transformation protocols were followed in order to obtain the part. All initial, intermediate, and final products obtained throughout the 3A assembly were digested with EcoRI-HF following our digestion protocol and run in a 1.2% agarose gel to confirm that the constructs were of the right size (Figure 1).
However, no blue colonies were observed after 24 hs of incubation in agar and no blue pellet was obtained after centrifuging an overnight LB broth culture at 7000 rpm for 5 min. The reason for the lack of expression remains elusive.