Coding
LuxE

Part:BBa_K590061

Designed by: Chris Choe and David Zong   Group: iGEM11_Washington   (2011-09-15)

LuxE; standalone, E. Coli codon optimized

This part consists of a synthesized version of the luxE gene from the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases Cambridge 2010 LuxBrick]. It is optimized to have cloning-friendly GC content and is also codon-optimized for expression in Escherichia coli.

Usage and Biology

The [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has tried to use this part along with Lux C and Lux D to engineer alternative routes for fatty aldehyde production.
Washington luxcde diagram.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 115


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Categories
Parameters
None