Composite
1C2

Part:BBa_K590054

Designed by: Lei Zheng, Ben Mo, Elaine Lai, Juhye An   Group: iGEM11_Washington   (2011-09-15)

RrD_N25M-PSB1C3-High constitutive

This part consists of Acyl-ACP Reductase, ribosomal binding site, mutant Aldehyde Decarbonylase under the control of high consititutive promoter for alkane production.

Usage and Biology

When wild type Aldehyde Decarbonylase is co-expressed with Acyl-ACP Reductase, odd chain length alkanes is produced from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the 2011 University of Washington iGEM Team. When mutant ADC and AAR are co-expressed, alkane production profile varies. For more information, refer to the [http://2011.igem.org/Team:Washington 2011 UW iGEM wiki].

Wild type ADC and AAR are co-expressed and GC-MS quantification of this co-expression shows greater alkane production under optimized conditions.
Mutant ADCs are co-expressed with AARs under optimized conditions.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1584
    Illegal XhoI site found at 2366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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