Aldehyde Decarbonylase (ADC) - Synechococcus elongatus PCC7942
This part encodes an enzyme Aldehyde Decarbonylase (ADC) that removes the carbonyl group (C=O) from a fatty aldehyde, yielding an alkane one carbon shorter than the original aldehyde and a molecule of formate. Works together with Acyl-ACP Reductase (AAR) to produce odd chain length alkanes. This part is an important component of the PetroBrick, which generates alkanes using AAR and ADC.
Usage and Biology
When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the 2011 University of Washington iGEM Team.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21INCOMPATIBLE WITH RFCIllegal XhoI site found at 730
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC