Composite
AAR1C3

Part:BBa_K590027

Designed by: Lei Zheng   Group: iGEM11_Washington   (2011-09-15)

AAR-PSB1C3-High consititutive

This part consists of Acyl-ACP Reductase in PSB1C3 high consititutive vector. This was an important negative control in testing the PetroBrick - which consists of Acyl-ACP Reductase and another part from Synechococcus elongatus, Aldehyde Decarbonylase.


Usage and Biology

Fatty Acid Biosynthesis.jpg

This part produces AAR, which catalyzes the firststep in the conversion of cellular fatty acyl-ACPs to alkanes. AAR reduces the fatty acyl-ACP to an aldehyde. To generate alkanes, this part is paired with ADC in the PetroBrick.

GC plot showing that alkanes are only produced when both ADC and AAR are expressed. The red peak corresponds to AAR alone - which generates large amounts of aldehyde, which are then reduced to the corresponding alcohol by cellular alcohol dehydrogenases.






















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1584
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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