Coding

Part:BBa_K590020

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)

mamJ, from Magnetospirillum magneticum

J gene with Bgl construction We made mamJ that is in gibson reaciton friendly(with Bgl construction) and used it to fuse with pGA1C3.


Usage and Biology

Difference between mamJ gene in Biobrick fashion and mamJ in Gibson Assembly fashion...(picture here) Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2051
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2051
    Illegal BglII site found at 2066
    Illegal BamHI site found at 1
    Illegal XhoI site found at 16
    Illegal XhoI site found at 1035
    Illegal XhoI site found at 1927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2051
    Illegal NgoMIV site found at 2298
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2491


[edit]
Categories
Parameters
n/amamJ, from Magnetospirillum magneticum