Coding

Part:BBa_K590019

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)

mamQRBSTUV in vector pGA3K3

This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 7 genes mamQRB, mamSTU, mamV, in forms of 3 inserts and is in pGA3k3 plasmid backbone. All the genes were found essential for magnetosome formation in Magnetospirillum magneticum strain AMB-1 on a pGA3k3 plasmid backbone.


Usage and Biology

Made by Gibson Cloning method instead of Standard Biobrick method, this part is in low copy backbone and it belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit], known as the "last part of the full assembly".

IGEM11 QRBSTUV partregistry.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3783
    Illegal EcoRI site found at 5317
    Illegal PstI site found at 1813
    Illegal PstI site found at 2553
    Illegal PstI site found at 4065
    Illegal PstI site found at 5140
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3783
    Illegal EcoRI site found at 5317
    Illegal PstI site found at 1813
    Illegal PstI site found at 2553
    Illegal PstI site found at 4065
    Illegal PstI site found at 5140
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3783
    Illegal EcoRI site found at 5317
    Illegal BglII site found at 990
    Illegal BglII site found at 1005
    Illegal BglII site found at 1574
    Illegal XhoI site found at 1067
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3783
    Illegal EcoRI site found at 5317
    Illegal PstI site found at 1813
    Illegal PstI site found at 2553
    Illegal PstI site found at 4065
    Illegal PstI site found at 5140
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3783
    Illegal EcoRI site found at 5317
    Illegal PstI site found at 1813
    Illegal PstI site found at 2553
    Illegal PstI site found at 4065
    Illegal PstI site found at 5140
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 1365
    Illegal NgoMIV site found at 1973
    Illegal NgoMIV site found at 3121
    Illegal NgoMIV site found at 3225
    Illegal AgeI site found at 1631
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4160
    Illegal SapI.rc site found at 5221


[edit]
Categories
Parameters
n/amamQRBSTUV in vector pGA3K3