Coding
mamMN

Part:BBa_K590004

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)

mamMN, from Magnetospirillum magneticum

This part includes genes mamM and mamN, which were extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].

Usage and Biology

The gene numbers of mamM and mamN aer AMB0967 and AMB0968 respectively. The part is 2323bp in length. MamMN was reported to be involved in iron transport, magnetite nucleation, or establishment of the proper chemical environment for magnetite synthesis in magnetosomes.

Below is the gel image of mamMN(2323bp):

1kb ladder (left), mamMN (right)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 288
    Illegal PstI site found at 74
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 288
    Illegal NheI site found at 1331
    Illegal PstI site found at 74
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 288
    Illegal BglII site found at 361
    Illegal BamHI site found at 1628
    Illegal BamHI site found at 2148
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 288
    Illegal PstI site found at 74
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 288
    Illegal PstI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 477
    Illegal SapI.rc site found at 56


[edit]
Categories
Parameters
n/amamMN, from Magnetospirillum magneticum