Part:BBa_K587010

Tast -> Key sequence for low concentration mechanism

This sequence forms part of the group of pieces directed to the expression of a concentration mechanism developed by our team ITESM_MEXICO 2011. The activation of gene expression is achieved by transcribing a non coding RNA (taRNA) produced by a second promoter (the same promoter dependant on the concentration amount). This mRNA targets the crRNA (either crstRNA or crwkRNA) specifically. In this case this key targets the crstRNA. The tastRNA is designed to hybridize the stem-loop created by the crstRNA and unfolds the loop exposing the Ribosome Binding Site (RBS) and allowing translation. The interaction between tastRNA and crstRNA undergo a linear-loop interaction that exposes the RBS followed by the activation of the reporter protein (CFP).

It has the same mechanism of action proposed by the team of British Columbia of 2009. Part: BBa_K206031

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]