Part:BBa_K587009

Crxwk -> Lock sequence for high concentration mechanism

This sequence forms part of the group of pieces directed to the expression of concentration mechanism developed by our team ITESM_MEXICO 2011. This part is based in the work of Isaacs and its team for the lock and key mechanism and in the work of the team of British Columbia University of 2009. This DNA sequence acts as a lock for the high arabinose concentration regulation path. In the construct the Pbad weak can activate the expression of the reporter protein CFP (Cyan Fluorescent Protein) for high sensitive expression, but there is a previous step where there is the first lock (crxst) is already inhibited by the action of the antisense key (itast); This second lock is activated by the expression of the Pbad weak promoter then it inhibits the expression of the reporter protein by joining the RBS and stopping the action of the mRNA polymerase to produce the mRNA and hence the protein. It has the same mechanism of action as the crxst lock. Part: BBa_K587008

Crxwk resulting transcripst (crwkRNA) blocks the recognition of the RBS by the ribosomal subunit (30s) by a cis system. This interaction prevents the translation of the repressor protein (CFP) A promoter can give the expression of the reporter gene. After transcription the mRNA shows a Ribosome Binding Site (RBS). The crwkRNA is complementary to the RBS and is inserted downstream of the promoter. And upstream the RBS. After transcription a stem-loop is formed at the 5' end of the mRNA, this mRNA blocks the ribosome binding and the translation by cis repression.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]