DNA

Part:BBa_K587003

Designed by: David Ignacio Maycotte Cervantes   Group: iGEM11_ITESM__Queretaro   (2011-09-28)


Tast -> Key to unlock crxst

This sequence acts as the key for the Crxst lock in the low concentration construct. It unlocks the Crxst, in order to activate following mechanism. The key must be at the same concentration than the lock, with the same promoter there is the activation of the key (tast)

This key has an interaction with the sequence of the lock, inhibiting its action so the gene can be expressed, this event occurs when the key (tast) has a chemical interaction with the lock (crxst)blocking its action and letting the translation of the reporter protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None