DNA

Part:BBa_K587002

Designed by: David Ignacio Maycotte Cervantes   Group: iGEM11_ITESM_Mexico   (2011-09-28)


Crxst -> Lock sequence for concentration mechanism

This part is based in the work of Isaacs and its team for the lock and key mechanism and in the work of the team of British Columbia University of 2009. This DNA sequence acts as a lock for the low arabinose concentration regulation path. In the construct the Pbad strong can activate the expression of the reporter protein GFP, but there is a previous step where there is the first lock (crxst); This first lock inhibits the expression of the reporter protein by joining the RBS and stopping the action of the mRNA polymerase to produce the mRNA and hence the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None