Coding

Part:BBa_K587001

Designed by: David Ignacio Maycotte Cervantes   Group: iGEM11_ITESM__Queretaro   (2011-09-28)


RecA protein coding sequence

Designed by Antonio Villarreal Larrauri and David Maycotte Cervantes. RecA protein that activates the expression of the promoters for arabinose concentration by reducing the activity of the lambda repressor.

RecA is a protein used for the cleavage of protein lambda. It has shown that it has a cleavage activity when its binded to lambda repressor (Sauer et al, 1981). This part represents a good form to inhibit the activity of lambda repressor in construct where lambda repressor and the lambda operators appear.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 127
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None