Generator

Part:BBa_K584024

Designed by: Bakul Jitendra Vinchhi, Sarah Veugelen, Katrien Vandermeeren, Yana Hoorne   Group: iGEM11_KULeuven   (2011-09-17)

Ice Nucleating Protein Generator (RBS + InaZ [K584021] + Terminator)

This part is the Ice Nucleating protein generator. It is composed of the ribosome binding site (RBS), ice nucleating protein gene inaZ and the two terminators BBa_B0010 BBa_B0012.

The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. inaZ is the gene responsible for producing the ice nucleation protein.

We would like to thank Prof. Dr. Gregory Gloor , for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene inaZ.

To know more about the inaZ, it is suggested to read this article describing the usage of pUC1813ICE with the inaZ.

This part was characterized qualitatively and quantitatively.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 664
    Illegal NgoMIV site found at 2176
    Illegal NgoMIV site found at 2320
    Illegal NgoMIV site found at 2584
    Illegal NgoMIV site found at 2728
    Illegal NgoMIV site found at 2776
    Illegal NgoMIV site found at 2896
    Illegal AgeI site found at 435
    Illegal AgeI site found at 1840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 610
    Illegal BsaI.rc site found at 3356
    Illegal SapI.rc site found at 256


Media:Example.ogg

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