DNA
Part:BBa_K582003
Designed by: Mitesh Agrawal Group: iGEM11_GeorgiaTech (2011-09-28)
Cas 9 + Repeat/Spacer Region
The Spacer/Repeat Region part has been extracted from the Streptococcus Thermophilus LMD9 genome sequence. If inserted along with the CRISPR protein system and sigma factor 70, we hypothesize that the spacer/repeat region should express itself and make bacteria resistant to which the spacer region is homologous.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 4698
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 4698
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 4698
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 4698
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3202
Illegal BsaI.rc site found at 4173
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Categories
Parameters
None |