DNA

Part:BBa_K582003

Designed by: Mitesh Agrawal   Group: iGEM11_GeorgiaTech   (2011-09-28)


Cas 9 + Repeat/Spacer Region

The Spacer/Repeat Region part has been extracted from the Streptococcus Thermophilus LMD9 genome sequence. If inserted along with the CRISPR protein system and sigma factor 70, we hypothesize that the spacer/repeat region should express itself and make bacteria resistant to which the spacer region is homologous.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 4698
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 4698
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 4698
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 4698
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3202
    Illegal BsaI.rc site found at 4173


[edit]
Categories
Parameters
None