Coding

Part:BBa_K582001

Designed by: Mitesh Agrawal   Group: iGEM11_GeorgiaTech   (2011-09-28)


Cas9 genome from the Streptococcus Thermophilus LMD-9 strain

Cas9 genome is a part of the CRISPR sequence existing in the Strep. Thermophilus LMD-9 strain. As referred by the Barrangou 2011 paper, cas9 is the only essential cas protein required for the CRISPR mechanism to express. We propose a biobrick with the cas9 site present in it as well as the promoter sigma factor 70 for its expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3202


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Parameters
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