Generator

Part:BBa_K581014

Designed by: Weiyue Ji, Dingqiao Wen   Group: iGEM11_Peking_S   (2011-09-26)

lux repressible promoter-rbs-GFP-ssrA-terminator

GFP + ssrA degradation tag behind a lux repressible promoter

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 953
    Illegal SpeI site found at 808
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 808
    Illegal SpeI site found at 954
    Illegal PstI site found at 968
    Illegal NotI site found at 961
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 954
    Illegal SpeI site found at 808
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 808
    Illegal SpeI site found at 954
    Illegal PstI site found at 968
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 703


[edit]
Categories
Parameters
n/alux repressible promoter-rbs-GFP-ssrA-terminator