Coding

Part:BBa_K581012

Designed by: Weiyue Ji, Hanchi Lin, Dingqiao Wen, Handuo SHI   Group: iGEM11_Peking_S   (2011-09-26)

pqrr-rbs-T7ptag-terminator

T7ptag behind pqrr.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

▪ promoter qrr was amplified by DNAWorks


References

  • [http://nar.oxfordjournals.org/content/30/10/e43.full.pdf+html] David M. Hoover, Jacek Lubkowski. DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis. Nucl. Acids Res 30 (10): e4 (2002).
[edit]
Categories
Parameters
None