Generator

Part:BBa_K581009

Designed by: Handuo SHI, Dingqiao Wen   Group: iGEM11_Peking_Signaling   (2011-09-26)

pqrr-GFP ssrA degradation tag

GFP behind promoter qrr


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 936
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 936
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 936
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 936
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 831


Design Notes

▪ promoter qrr was amplified by DNAWorks


References

  • [http://nar.oxfordjournals.org/content/30/10/e43.full.pdf+html] David M. Hoover, Jacek Lubkowski. DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis. Nucl. Acids Res 30 (10): e4 (2002).
[edit]
Categories
Parameters
n/apqrr-GFP