Regulatory

Part:BBa_K578004

Designed by: Ethan Ward   Group: iGEM11_Arizona_State   (2011-09-28)

Bacillus halodurans C-125 CRISPR leader sequence

This is a putative leader sequence for the CRISPR III locus in Bacillus halodurans C-125.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Confirmation of Part Length

PCR of BBa_K578004 using Primers VF2 (BBa_G00100) and VFR (BBa_G00101) from pSB1C3. The part is 235bp plus 42bp for the Standard BioBrick prefix and suffix. Note: The primers add approximatellly 200bp to the BioBrick's size, thus BBa_K578004 should appear at ~477bp (Band appears larger than expected and requires sequence confirmation).

ASU Bh Leader.png


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Categories
Parameters
None