Reporter

Part:BBa_K577882

Designed by: Alberto Purwada   Group: iGEM11_BU_Wellesley_Software   (2011-09-11)


pC+AraC+pBad+RFPc

This reporter part is a combination of a constitutive promoter, AraC gene, a pBad promoter (inducible with arabinose), and a RFP gene. The purpose of this part is to provide the user with a simple, but complete, inducible RFP genetic device. Adding arabinose with any concentration higher than 0.01 mM seems to cause a significant expression of the RFP gene. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. This part can be inserted in a cell line that does not contain any AraC in its genome, which is important if the user is not using Top 10 cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1833
    Illegal AgeI site found at 1945
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None