Reporter

Part:BBa_K576012

Designed by: Joon Hyuk Hong   Group: iGEM11_Waterloo   (2011-06-20)


J23101-RBS-GFP1-Lox-GFP2-Transcription Terminator

In this negative control, GFP1 and GFP2, followed by a transcriptional terminator, flank RFC10 resulting in a stop-codon-containing scar. No fluorescence is expected for this component (background) because translation is interrupted.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 541
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 781
    Illegal SapI site found at 598


[edit]
Categories
Parameters
None