Part:BBa_K575046
This construct was developed by Northwestern's 2011 iGEM team as part of a Pseudomonas Aeruginosa detector. The device is designed to fluoresce with GFP in the presence of PAI2 (C4-HSL), one of the Pseudomonas quorum sensing molecules. The promoter in front of GFP is activated by the combination of PAI2 from the environment and the RhlR receptor (produced by this construct). This promoter was taken from a region of the Pseudomonas genome known to be activated by RhlR/PAI2. As the graph shows, addition of the autoinducer produces a significant difference in fluorescence compared to the controls. Thus, our construct is successful both in production of RhlR and transcription of GFP in the presence of PAI2.
Genomic RhlR/PAI2 Inducible Promoter + RBS (B0034) + GFP, Cons. Promoter + RBS (B0034) + RhlR
Continuous expression of RhlR (with RBS B0034), coupled with a RhlR/PAI2 (C4-HSL) inducible promoter, RBS (Part B0034), and a GFP reporter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1282
Illegal NheI site found at 1305
Illegal NotI site found at 304 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1576
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 357
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2051
Illegal BsaI.rc site found at 1191
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