Composite

Part:BBa_K575034

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)

RhlR/PAI2 Inducible Promoter + RBS (B0030) + RFP + Constitutive Promoter + RBS (B0030) + RhlR

Continuous expression of RhlR (with RBS B0030), coupled with a RhlR/PAI2 (C4-HSL) inducible promoter, RBS (Part B0030), and an RFP reporter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 803
    Illegal NheI site found at 826
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 637
    Illegal AgeI site found at 749
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1575


[edit]
Categories
Parameters
None