Composite

Part:BBa_K575032

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)

This construct was developed by Northwestern's 2011 iGEM team as part of a Pseudomonas Aeruginosa detector. The device is designed to fluoresce with GFP in the presence of PAI2 (C4-HSL), one of the Pseudomonas quorum sensing molecules. The promoter in front of GFP is activated by the combination of PAI2 from the environment and the RhlR receptor (produced by this construct). As the graph shows, addition of the autoinducer produces a significant difference in fluorescence compared to the controls. Thus, our construct is successful both in production of RhlR and transcription of GFP in the presence of PAI2.

Rhlp-30-gfp cp-30-rhlr.jpg

RhlR/PAI2 Inducible Promoter + RBS (B0030) + GFP + Constitutive Promoter + RBS (B0030) + RhlR

Continuous expression of RhlR (with RBS B0030), coupled with a RhlR/PAI2 (C4-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 817
    Illegal NheI site found at 840
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1114
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1589
    Illegal BsaI.rc site found at 726


[edit]
Categories
Parameters
None