Composite

Part:BBa_K575027

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)

LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP, Constitutive Promoter + RBS (B0034) + LasR

This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter with an RBS and an RFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 907
    Illegal NheI site found at 930
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1283
    Illegal AgeI site found at 741
    Illegal AgeI site found at 853
    Illegal AgeI site found at 1480
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None