Reporter

Part:BBa_K572003

Designed by: Avaneesh T   Group: iGEM11_IIT_Madras   (2011-09-17)


OmpR Promoter_RFP Generator

In conditions of high osmolality, OmpR is phosphorylated and it binds to the three operating sites of the promoter (BBa_R0082) and activates transcription. This part is used as a control for our experiments dealing with increasing recombinant protein production using proteorhodopsin. It is also an indication of the amount of protons present in the periplasmic space.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 692
    Illegal AgeI site found at 804
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None