Part:BBa_K571006

R0011+acsCD/pSB1C3

The biobrick is composite element, it is the combination of BBa_K571001 and BBa_K571003. The promoter binding to the upstream of acsCD gene, IPTG as the inducer, activates the acsCD gene to synthesize protein.

The sequence of acsCD and promoter is amplified by PCR, followed by enzyme digestion and ligation to bind the 2 sequences with the backbone-pSB1C3. The order of restriction enzyme is EcoRI, XbaI and SpeI.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3233
  Illegal PstI site found at 143
  Illegal PstI site found at 329
  Illegal PstI site found at 779
  Illegal PstI site found at 2615
  Illegal PstI site found at 2945
  Illegal PstI site found at 3071
  Illegal PstI site found at 4144
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3233
  Illegal PstI site found at 143
  Illegal PstI site found at 329
  Illegal PstI site found at 779
  Illegal PstI site found at 2615
  Illegal PstI site found at 2945
  Illegal PstI site found at 3071
  Illegal PstI site found at 4144
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3233
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3233
  Illegal PstI site found at 143
  Illegal PstI site found at 329
  Illegal PstI site found at 779
  Illegal PstI site found at 2615
  Illegal PstI site found at 2945
  Illegal PstI site found at 3071
  Illegal PstI site found at 4144
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3233
  Illegal PstI site found at 143
  Illegal PstI site found at 329
  Illegal PstI site found at 779
  Illegal PstI site found at 2615
  Illegal PstI site found at 2945
  Illegal PstI site found at 3071
  Illegal PstI site found at 4144
  Illegal NgoMIV site found at 662
  Illegal NgoMIV site found at 1738
  Illegal NgoMIV site found at 2858
  Illegal AgeI site found at 874
  Illegal AgeI site found at 2165
  
• 1000
  COMPATIBLE WITH RFC[1000]