Part:BBa_K571000

acsAB/pSB1C3

The acs operon in Gluconacetobacter hansenii is the mechanism that synthesize cellulose synthase, acsAB is one of the gene sequence. The acsAB will produce protein with 2 subunits; one is catalytic subunit which is able to transform UDP-glucose to UDP, another one would be the regulatory subunit of the cellulose synthase which will bind with cyclic di-GMP and activated. From the primer we design, the acsAB sequence is amplified from PCR from the genomic DNA of Gluconacetobacter hansenii, XbaI and SpeI is also added at the both end of primer as the cutting site, so that acsAB and pSB1C3 could easily bind to it.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 580
  Illegal EcoRI site found at 1981
  Illegal PstI site found at 736
  Illegal PstI site found at 1132
  Illegal PstI site found at 1972
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 580
  Illegal EcoRI site found at 1981
  Illegal PstI site found at 736
  Illegal PstI site found at 1132
  Illegal PstI site found at 1972
  Illegal NotI site found at 3147
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 580
  Illegal EcoRI site found at 1981
  Illegal BglII site found at 1136
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 580
  Illegal EcoRI site found at 1981
  Illegal PstI site found at 736
  Illegal PstI site found at 1132
  Illegal PstI site found at 1972
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 580
  Illegal EcoRI site found at 1981
  Illegal PstI site found at 736
  Illegal PstI site found at 1132
  Illegal PstI site found at 1972
  Illegal NgoMIV site found at 2355
  Illegal NgoMIV site found at 2395
  Illegal AgeI site found at 4527
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1069