Reporter

Part:BBa_K567026

Designed by: You Wang   Group: iGEM11_SJTU-BioX-Shanghai   (2011-10-28)

PT7-Luc-2x4AGG(30bp)

This biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator. 4 AGG codons and 2 GCG codons are inserted in TWO DIFFERENT places after the ATG start codon of wild type luciferase (BBa_I712019) with an interval of 30bp.

Related Biobrick

lacI-Ptrc-tRNAArg (BBa_K567001)

PT7-Luc-2x4AGG(30bp) (BBa_K567026)

PT7-Luc-3x4AGG(30bp+111bp) (BBa_K567027)



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 48
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 48
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 953


[edit]
Categories
Parameters
n/aPT7-Luc-2x4AGG(30bp)