Part:BBa_K567017

PT7-RFP-6AGG

RFP with 6 AGG-codon insertions after ATG under the control of T7 promoter and lac operator. The protein can be expressed successfully when co-transformed with PT7-TDRS (BBa_K567011) and tRNA(Asp)-AGG (BBa_K567012). Modified RFP exhibits lower red fluorescence brightness. This part is used to testify the function of PT7-TDRS (BBa_K567011).

Related Biobrick:

lacI-Ptrc-tRNA^Arg (BBa_K567001)

PT7-TDRS (BBa_K567011)

tRNA^Asp-AGG (BBa_K567012)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 983
  Illegal XbaI site found at 48
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 983
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 983
  Illegal XhoI site found at 845
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 983
  Illegal XbaI site found at 48
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 983
  Illegal XbaI site found at 48
  Illegal AgeI site found at 721
  Illegal AgeI site found at 833
  
• 1000
  COMPATIBLE WITH RFC[1000]