Part:BBa_K567002

sulA promoter-tRNA(Arg)

The tRNA(Arg) is under the control of sulA promoter induced by UV. tRNA expression is induced by 20-second UV exposure (the distance between the 20W lamp and the culture is 35cm) when the OD600 of the culture reaches 0.3.

Fig. the picture shows the change of luciferase activity before and after UV induction. As it is shown in the figure, reporter gene expression increased, indicating that tRNA is expressed after induction

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 177
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 177
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 177
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 177
  
• 1000
  COMPATIBLE WITH RFC[1000]