Part:BBa_K566031
E. coli MXGreen
Derived from Dr. Jeff Tabor JT2 E. coli strain, this part is a built-in red-light induction system in E. coli made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher.
It is proposed as a photo-chassis that could make useful tools in this field of light induction.
Integration was performed for the genes pcyA, ho1, Cph8 and a Mnt working as follows: Genes ho1 and pcyA are responsible for the chromophore synthesis. Cph8 codes for the chimaeric red-light receptor. These three genes are constitutively expressed. Mnt repressor is expressed from pOmpC promoter, which stops being induced in presence of red-light. It is therefore used as a NOT-gate to regulate expression from pMnt.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1957
Illegal EcoRI site found at 4101
Illegal SpeI site found at 2653
Illegal SpeI site found at 4196 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1957
Illegal EcoRI site found at 4101
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 963
Illegal NheI site found at 986
Illegal NheI site found at 1895
Illegal NheI site found at 1918
Illegal NheI site found at 2809
Illegal SpeI site found at 2653
Illegal SpeI site found at 4196 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1957
Illegal EcoRI site found at 4101
Illegal BamHI site found at 2354
Illegal XhoI site found at 5118 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1957
Illegal EcoRI site found at 4101
Illegal SpeI site found at 2653
Illegal SpeI site found at 4196 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1957
Illegal EcoRI site found at 4101
Illegal SpeI site found at 2653
Illegal SpeI site found at 4196
Illegal NgoMIV site found at 386
Illegal AgeI site found at 4136 - 1000COMPATIBLE WITH RFC[1000]
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