Part:BBa_K566019

E. coli MxRed

Photochassis capable to respond to red light. With Mnt expression under the control of pOmpC which is directed to an Mnt repressible promoter.

Usage and Biology

Derived from Dr. Jeff Tabor JT2 E. coli strain, this part is a built-in red-light induction system in E. coli made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher.

It is proposed as a photo-chassis that could make useful tools in this field of light induction.

Integration include the genes pcyA, ho1, Cph8 and a Mnt working as follows: Genes ho1 and pcyA are responsible for the chromophore synthesis. Cph8 codes for the chimaeric red-light receptor(1). These three genes are constitutively expressed. Mnt repressor is expressed from pOmpC promoter, and the red light stops its induction. It is therefore used as a NOT-gate to regulate expression from pMnt.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 167
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 3043
  Illegal NheI site found at 3066
  Illegal NheI site found at 3999
  Illegal NheI site found at 4022
  Illegal PstI site found at 167
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 167
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 167
  Illegal NgoMIV site found at 3422
  
• 1000
  COMPATIBLE WITH RFC[1000]