Composite

Part:BBa_K565002

Designed by: Juan Ricardo Ortiz Meneses   Group: iGEM11_Valencia   (2011-09-17)

Colicin H gene cluster

Colicin H protein is produced by Escherichia coli CA 58 strain. Its specific activity making holes in bacterial membranes need the presence of Fiu protein in the outer membrane of target cells in order to recognize them, and use the TonB/ExbB, D translocation route to slip in the cell and lyse it, being lethal for related strains of E. coli. To disinfect the contaminated water in our project, we used Colicin H to kill pathogen cells (in our case they were E. coli DH5alpha strain) by membrane lysis.

We got this part from UK's Health Protection Agency strain collection.

Bibliography

Cascales E. et al. Colicin Biology. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, Mar. 2007, p. 158–229 Vol. 71, No. 1.

Patzer S. I. et al. The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN. Microbiology (2003), 149, 2557–2570.

Bradley D. E. 1991. Colicins G and H and their host strains. Can. J. Microbiol. 37: 751-757.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 932
    Illegal EcoRI site found at 5029
    Illegal EcoRI site found at 5780
    Illegal EcoRI site found at 6325
    Illegal EcoRI site found at 8784
    Illegal EcoRI site found at 10848
    Illegal XbaI site found at 7968
    Illegal SpeI site found at 3718
    Illegal SpeI site found at 3730
    Illegal PstI site found at 22
    Illegal PstI site found at 1637
    Illegal PstI site found at 6526
    Illegal PstI site found at 6535
    Illegal PstI site found at 7077
    Illegal PstI site found at 9776
    Illegal PstI site found at 9816
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 932
    Illegal EcoRI site found at 5029
    Illegal EcoRI site found at 5780
    Illegal EcoRI site found at 6325
    Illegal EcoRI site found at 8784
    Illegal EcoRI site found at 10848
    Illegal SpeI site found at 3718
    Illegal SpeI site found at 3730
    Illegal PstI site found at 22
    Illegal PstI site found at 1637
    Illegal PstI site found at 6526
    Illegal PstI site found at 6535
    Illegal PstI site found at 7077
    Illegal PstI site found at 9776
    Illegal PstI site found at 9816
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 932
    Illegal EcoRI site found at 5029
    Illegal EcoRI site found at 5780
    Illegal EcoRI site found at 6325
    Illegal EcoRI site found at 8784
    Illegal EcoRI site found at 10848
    Illegal BglII site found at 3540
    Illegal BglII site found at 7368
    Illegal BglII site found at 7531
    Illegal BamHI site found at 8777
    Illegal BamHI site found at 11515
    Illegal BamHI site found at 12976
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 932
    Illegal EcoRI site found at 5029
    Illegal EcoRI site found at 5780
    Illegal EcoRI site found at 6325
    Illegal EcoRI site found at 8784
    Illegal EcoRI site found at 10848
    Illegal XbaI site found at 7968
    Illegal SpeI site found at 3718
    Illegal SpeI site found at 3730
    Illegal PstI site found at 22
    Illegal PstI site found at 1637
    Illegal PstI site found at 6526
    Illegal PstI site found at 6535
    Illegal PstI site found at 7077
    Illegal PstI site found at 9776
    Illegal PstI site found at 9816
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 932
    Illegal EcoRI site found at 5029
    Illegal EcoRI site found at 5780
    Illegal EcoRI site found at 6325
    Illegal EcoRI site found at 8784
    Illegal EcoRI site found at 10848
    Illegal XbaI site found at 7968
    Illegal SpeI site found at 3718
    Illegal SpeI site found at 3730
    Illegal PstI site found at 22
    Illegal PstI site found at 1637
    Illegal PstI site found at 6526
    Illegal PstI site found at 6535
    Illegal PstI site found at 7077
    Illegal PstI site found at 9776
    Illegal PstI site found at 9816
    Illegal AgeI site found at 1107
    Illegal AgeI site found at 1927
    Illegal AgeI site found at 11896
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3841
    Illegal BsaI.rc site found at 10482
    Illegal SapI site found at 11591
    Illegal SapI.rc site found at 4727
    Illegal SapI.rc site found at 7415
    Illegal SapI.rc site found at 8335


[edit]
Categories
Parameters
n/aColicin H gene cluster