Regulatory

Part:BBa_K564021

Designed by: Sébastien Lemire   Group: iGEM11_DTU_Denmark   (2011-09-21)


aTC regulated promoter module: aTC--PoPS

This part contains the tet regulated promoter R0040 as well as the wild-type tetR gene organized opposite one another to allow creation of an autonomous tet regulatable promoter module. Indirect evidence suggests it does and works as expected (induced by anhydrotetracycline).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 848
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 838
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None